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Antibodies certain to the blocking peptide no longer bind into the epitope about the target protein. By evaluating the staining in the blocked antibody vs . the antibody on your own, you can see which staining is particular.
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This guidebook outlines further more achievable optimization of mobile and immunoassay parameters to make sure the best possible benefits are...
This guide outlines even more possible optimization of cellular and immunoassay parameters to guarantee the best possible final results are...
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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by equally antibodies...
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Blocking peptides are peptides that bind particularly towards the target antibody and block antibody binding. These peptide generally consists of the epitope recognized by the antibody.
Concentrate on Information and facts Cyclin E belongs to your hugely conserved cyclin household, whose associates are characterized by a extraordinary periodicity in protein abundance from the cell cycle. Cyclins perform as regulators of CDK kinases. Different cyclins show unique expression and degradation patterns which add on the temporal coordination of each mitotic event. Cyclin E varieties a fancy with and functions for a regulatory subunit of CDK2, whose action is get more info required for cell cycle G1/S changeover. This protein accumulates with the G1-S phase boundary and is particularly degraded as cells progress as a result of S section.
Overexpression of the gene is noticed in lots of tumors, which results in chromosome instability, and therefore might lead to tumorigenesis. This protein was discovered to affiliate with, and be involved with, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in mobile-cycle controlled histone gene expression and plays a significant part in promoting cell-cycle development within the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinctive isoforms, have already been explained. Two additional splice variants have been claimed but in-depth nucleotide sequence data just isn't however offered.
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